Trypanosomatid infections in non-human primates of Costa Rica
Antony Solorzano MoralesEl Gaff
Antony Solorzano Morales, Connie Tien, Sergio Alfaro Porras, Gaby Dolz
Neotropical non-human primates are frequently infected with a variety of trypanosomes, including Trypanosoma cruzi, which causes Chagas disease, an important zoonotic disease in Latin America. The objective of this study was to determine antibodies against T. cruzi and to characterize by molecular methods trypanosomes present in non-human primates (NHP) of Costa Rica. 201 blood samples recollected between 2011-2016 from wild (49) and in captivity living (152) NHP were analyzed by molecular (polymerase chain reaction [PCR] and sequencing) and serological (indirect hemagglutination [IHA]) methods. A total of 21 (10%) samples were positive in PCR, sequence and BLAST analysis of the high variable region of the small subunit ribosomal RNA gene determined them as Trypanosoma minasense. The presence of this parasite was determined in the four species of monkeys in the country (Alouatta palliata n=5, Ateles geoffroyi n=6, Cebus imitator n=8 and Saimiri oerstedii n=1); coming from different regions (Alajuela province n=8, Limón n=4, Puntarenas n=9) and both lifestyle (wild=3, captivity=18). All sera (123) analyzed reacted negative in IHA. This represent the first molecular report of T.minasense infecting non-human primates from Costa Rica. Future studies about the biology of this parasite and the existence of other trypanosomatid species, including T.cruzi, in non human primates of Costa Rica are required.