Draft genome sequence of a multi-drug resistance and highly virulent Salmonella enterica ser. Infantis ST32 producing CTX-M-65 isolated from a Magellanic Horned Owl (Bubo magellanicus) in Chile

Danny Fuentes-Castillo, Mariella Farfán-López, Fernanda Esposito, Quézia Moura, Brenda Cardoso, Maria E. Muñoz, Louise Cerdeira, José L. Catão-Dias, Daniel González-Acuña, Nilton Lincopan

Salmonella enterica serovar Infantis has significant adaptation capacity in human and poultry. In this study, we present the first draft genome sequence of a S. Infantis isolated from a wild bird. In February 2018, a Magellanic Horned Owl (Bubo magellanicus) was admitted in a wildlife rescue and rehabilitation center (WRRC) in Southern Chile (36°35’38.94″S; 72°5’2.79″O), malnourished, depressed, with moderate dehydration and blindness in the right eye. Cloacal samples were streaked onto MacConkey agar plates supplemented with ceftriaxone and enterobacterial isolates were identified by MALDI-TOF MS system. The whole genome was sequenced using an Illumina NextSeq platform and was assembled using Velvet v.1.2.10, and data analysis was carried out using available tools from the Center of Genomic Epidemiology. A S. Infantis ST32 (CDR398A) was isolated from this owl, that displayed a multidrug resistance to ?-lactams (blaCTX-M-65), aminoglycosides (aph(3′)-Ia, aph(4)-Ia, aadA1, aac(6′)Iaa, aac(3)-Iva), phenicols (floR), (sul1), tetracyclines (tetA) and diaminopyrimidines (dfrA14). Additionally, chromosomal point mutations were detected in gyrA (Asp87Tyr) gene, which confer resistance to fluoroquinolones. Heavy metal resistance genes to zinc, magnesium, copper, molybdenum, iron, mercury and manganese, and resistance genes to quaternary ammonium compounds were detected. Virulence genes associated to type III secretion system, yersiniabactin, Non-fimbrial adhesion factors, antimicrobial resistance peptide, fimbriae and curli fibers were detected. To evaluate the virulence potential of CDR398A, an in vivo experiment was carried out with the Galleria mellonella infection model, that confirmed a high-virulence background of CDR398A. Interestingly, the resistome of CDR398A is similar to strains of S. Infantis carrying blaCTX-M-65 isolated from human and chicken samples from United States. To the best of our knowledge, this is the first draft genome sequence of a multidrug resistance blaCTX-M-65-carrying S. Infantis recovered from a wild bird, highlighting the importance of applying a global One Health human-animal-environment perspective to understand and combat the antimicrobial resistance.

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