Detection and characterization of Chelonid alphaherpesvirus 5 in mydas chelonian secretions in the Brazilian Atlantic Coast
Marco Aurélio GattamortaEl Gaff
Marco Aurélio Gattamorta
The epidemiological approach considers that the disease in the population is a dynamic phenomenon and its propagation depends on the interaction between the exposure and the susceptibility of individuals and constituent groups of a given population to the factors determining the presence of the disease. The mechanisms of transmission of an infectious agent may be related to the number of infectious particles, the agent's ability to remain in the environment, transmission routes, and aspects related to time, place and individual. The detection of Chelonid alphaherpesvirus 5 (ChHV5), the primary agent of fibropapillomatosis (FP), have recently been detected in secretions, indicating a possible route of infection or reinfection. Considering epidemiological principles, this work was developed with the aim of understanding of the dispersion of ChHV5, evaluating the presence of the virus in secretions in 3 feeding areas of Chelonia mydas in the Brazilian coast, whose prevalence of FP is relatively different : Fernando de Noronha - RN (0.14%), Ubatuba - SP (10.43%) and Vitória - ES (21.06%, reaching 44.1% at industrial effluent disposal sites) as well as characterize ChHV5 in secretions. Oral and ocular swabs were colletect from individuals of Fernando de Noronha (40), Ubatuba (25) and Vitória (31). The total DNA extracted was subjected to PCR for the UL-18, UL-22, UL-27, UL-30 target genes. A 460 bp DNA polymerase sequences were analyzed. Positive secretions were detected in Fernando de Noronha (1) and in Ubatuba(5), and not detected in Vitória. Sequence analysis revealed the presence of a ChHV5 haplotype with similarity to previously characterized haplotypes in Atlantic. No correlation was found between the prevalence of FP and the presence of the virus in the secretions. These results indicate that there may be numerous mechanisms related to virus dispersion and FP prevalence can not be correlated with the elimination of the pathogen.